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֪R(sh)c(din)Y(ji)

r(sh)g2022-09-14 12:02:31 Y(ji) ҪͶ

֪R(sh)c(din)Y(ji)

Y(ji)ǰһ(g)r(sh)εČW(xu)(x)rM(jn)һȫϵy(tng)ĿY(ji)ʹ҂Ч׌҂һW(xu)(x)Y(ji)ɡô֪Y(ji)ΌСʹij֪R(sh)c(din)Y(ji)Уgӭxϣ܉ϲg

֪R(sh)c(din)Y(ji)

֪R(sh)c(din)Y(ji) 1

(yng)((g)w(du)Ĵ̼(hu)a(chn)һ(yng)(yng)DŽ(dng)B(ti)^)cm(yng)((yng)ԣҲoB(ti)m(yng)(dng)ֲıo(h)ɫ)ɻzQ

﹤̰󲿷֣քeǻ򹤳:(x)(μg(sh))l(f)͹ø̡

Ĺ԰ͬ|(zh)A(ch)(Ԫ)NNRNADNAнY(ji)(gu)ʽY(ji)(gu)(Ǿa^(q);a^(q))zܴa

Ԫغռ(x)rĶٵքeOCHNPS(x)ԪC

wПoC(j)}ĹܺãȱF(do)¼t(x)\(yn)ݔ½wF(xin)Sּ(x)(dng);ȱF(do)ؚѪwF(xin)Sw(dng)áΘ(gu)ɏ(f)s

ֲ:(x)е(ch)|(zh)քeǣ֬ۡ|(zh);(dng):(x)еҪ(ch)|(zh)Ҫ֬͵|(zh)^(q)ֱԴҪԴ(ch)ԴԴ

|(zh)Y(ji)(gu)ԭ(4(g))DNAY(ji)(gu)ԭ(3(g))DNAY(ji)(gu)(wn)ԭ(3(g))

(x)С΢ˮƽR¿ɿֱС0.2΢׵ļ(x)΢Y(ji)(gu)Сļ(x)֧ԭw

|(zh)ĻԪCHONSԪ;ĻԪCHONPPԪ;Ѫt׵ԪCHONFe~GصԪCHONMgԪCHON;V|(zh)Ԫص֬

ԭ˼(x)c(din)ТٟoĤʢڟoȾɫwۃHкwܼ(x)ڳɷľǢzѭҎ(gu)ɢރHеĿz׃ǻͻ׃ߟoĤϵy(tng)Y(ji)(gu)a^(q)Bm(x);(dng)t(x)o(x)˺;wM(jn)Пoȡ(x)Ĥĺòϡ

(ni)|(zh)W(wng)Ĥϵy(tng)c(x)ĤB(ni)cӺĤB߀cwĤB(du)|(zh)M(jn)ۯBMbǻȼӹγɾĤС\(yn)ݔ?sh)ߠwM(jn)һӹͷ

ڵпwɔ_(ǵ)øԭȍuL(zhng)ء(jng)^ĤԼ(x)Y(ji)(gu)Ѓ(ni)|(zh)W(wng)ߠwͼ(x)Ĥ

N(x)к˻Ҫȏ(f)ƽ֣|(zh)SC(j)ȷֻŷֳɼ(x)zͼ(x)|(zh)zɷNʽ(x)vδֻֻ˥ѴΔ(sh)Խļ(x)ԽL(zhng)(x)˥̓(ni)ͬõĽY(ji)

(x)ֻČ(sh)|(zh)ǻxԱ_(d)D(zhun)ˮƽɻɂ(c)Ǿa^(q){(dio)صġ

(x)ȫָѷֻĵļ(x)аl(f)ĝ(j)(dng):(x)ȫԴСɷ֞ȫԼ(x)((dng)̥(x))(ԭc߼(x))(Ѫɼ(x));(j)ֲ:(x)_(d)ȫԴСǣֳܾ(x)w(x);ȫԵ|(zh)A(ch)Ǽ(x)(ni)бNȫz|(zh)

Ӱøٷ(yng)ٶȵøȡضȡAȵʹø׃ԵǏ(qing)(qing)Aߜ؄(dng)w(ni)øĻԲضӰ-ømض60ҡ

򹤳̵Ĺøԃ(ni)øDNABø(cI);(x)̵ĹøwSø͹zø(@ԭ|(zh)wr(sh)m˝ȵҺCȝBo(h)ԭ|(zh)w)ȵø((dng):(x))

ATPǼ(x)(ni)ֱԴ|(zh)ڼ(x)(ni)cADP໥D(zhun)ܵ(dng)(dng)\(yn)ݔźͷڡ(yng)տs(jng)(do)늡ЙC(j)ϳɵ;ܵНB͸v;γATP(dng)Ǻú͹ⷴ(yng)

D(zhun)׃ɻSW(xu)ܕr(sh)ӹwˮKwoø(NADP+)هB(ti)~GaˮNADPHγɵăɂ(g)ʽ

߹ʵķ1L(zhng)Õr(sh)g(һ(ni)݆)2ӹe(ֲg)3߹Ч(ٶ)

B͸܄(ˮͪƾ)ͨ^͸ĤĔU(ku)ɢȑ(yng)QĦȣǰٷ֝

vˮ\(yn)ݔˮֵĄ(dng)Ҳ\(yn)ݔxӵĄ(dng);ֲˮĄ(dng)߀Ǹ;Ӱvõǜضȡ(ض)L(fng)ֲˮ(1%--5%)vȴr(sh)F(xin)ˮֲL(zhng)Ę(bio)־

Ҫ(j)ֲͬͬˮͬ(ji)M(jn)УɲÇιM(jn)M(jn)йȣ(ji)sˮ

oֳ(Ă(g))(dng)¡g(sh)ֲMB(yng)(dng)ֲָ̥g(sh)(yu)c(din)DZHă(yu)ԠֲMB(yng)ă(yu)c(din)ǣȡB(yng)ڶֳ̡ʸԄ(dng)(yng)Уٷֳ;oֲ;a(chn)ˎʳƷӄɫϡx;˹N;D(zhun)ֲ

ֲ(du)ˮֺ͌(du)xӵǃɂ(g)(du)(d)^̡עДٶȴС

w(ni)|(zh)āԴҪʳ֬ԴҪǸǡߵ׵D(zhun)

|(zh)w(ni)܃(ch)棬Ǽ(x)ĽY(ji)(gu)|(zh)͹|(zh)Դ|(zh)Óֽܷܡ|(zh)Ól(f)ڣ|(zh)z^ոz뵰|(zh)|(zh)ֽ⡢^I

wÿ횔zһĵ|(zh)ԭǵ|(zh)Ǽ(x)ĽY(ji)(gu)|(zh)͹|(zh);|(zh)w(ni)܃(ch);D(zhun)òγзNİ;|(zh)w(ni)ÿ춼¡(횰᣺ɫهKסi)

^(q)eȾɫwM(һ׷ͬԴȾɫw)ȾɫwM((g)w(ni)ȾɫwN͔(sh)Ŀ)ȾɫwM(磺Ѽ(x)ȾɫwMɣ22l+X);Ի(ˮ24)ĆαM12lȾɫwzϢ;˻M(wDNAyȫzϢ)ˆαM(24lȾɫwzϢ);

ЙC(j)xP(gun)ϵ(໥“(lin)ϵ໥Ƽs)D(zhun)(֬ܵ|(zh));D(zhun)Зl(ǹ(yng)D(zhun)׃?yu)֬ǿɴD(zhun)׃?yu)ֻ֬֬D(zhun)׃?yu)?;໥Ƽs(ֻǴxϵKr(sh)֬|(zh));ǴxĘм~

֪R(sh)c(din)Y(ji) 2

1.(x)W(xu)fĽ^

(1)(x)W(xu)fĄ(chung)ʼʩRǺʩ

(2)(x)W(xu)fҪc(din)ǣ(x)һ(g)ЙC(j)wһЄ(dng)ֲﶼɼ(x)l(f)ɼ(x)ͼ(x)a(chn)(gu);(x)һ(g)(du)(d)ĆλԼ֌(du)c(x)ͬMɵw;¼(x)ɏϼ(x)Юa(chn)

(3)(x)W(xu)fĄ(chung)(du)M(jn)Ҫxǣʾκ΄(dng)ֲɼ(x)(gu)ɵģĶf(dng)ֲ֮gһHP(gun)ϵ֮gHP(gun)ϵ(du)ʾM(jn)Ҫr(ji)ֵ

2.Nӵļ(x)

(4)Ȼϵy(tng)ČӴУ(x)M١ϵy(tng)(g)wNȺȺ䡢B(ti)ϵy(tng)Ȧ

(5)ֲϵy(tng)ӴЛ]Сϵy(tng)@(g)Ӵ

(6)ԭ˼(x)c˼(x)ı|(zh)^(q)eПoԺĤ޵ļ(x)

չ

ԭ˼(x)wo(x)ԭ缚(x)ļ(x)Ҫɷc|(zh)Y(ji)϶ɵĻ

ԭzϵ zҎ(gu);ֳ^У˻zϵ zҎ(gu)

Ȼl£ԭĿz׃ֻлͻ׃;Ŀz׃лͻ׃ؽMȾɫw׃

ԭ˼(x)缚(x)ҪԶѵķʽM(jn)з;˼(x)ķѷʽнzѡozp(sh)ѡ

(7)ܪ(d)Ĵxͷֳ^ֻĻ(x)M(jn)С

չ

ٲǼȲԭҲ

ڽMÿNĻλķNÓᣬķNǺ

۲B(yng)ֱB(yng)B(yng)?yn)鲡ķֳĻ?x)M(jn)

3.(x)Ĥϵy(tng)ĽY(ji)(gu)͹

(8)ò(dng).t(x)(sh)(yn)ܷxõļ(x)ĤѼ(x)ˮˮ(hu)M(jn)뼚(x)Ѽ(x)qƣ(x)(ni)|(zh)@ӾͿԵõļ(x)Ĥ

(9)(x)ĤҪ֬|(zh)͵|(zh)M߀

չ

ʹ(x)Ĥ|(zh)M(jn)ܵ|(zh)dw

ڼ(x)ĤcĤĻW(xu)MɴͬڲͬĤW(xu)|(zh)ĺвe(x)Ĥĺ(du)c(x)ĤҪࡣ

(10)(x)ĤĽY(ji)(gu)c(din)(dng)x͸^

(11)ڼ(x)Ĥһɼ(x)Ĥϵĵ|(zh)cY(ji)϶ɵǵDZDZc(x)R(sh)eP(gun)ϵͺƤ(x)ǵбo(h)͝(rn)á

(12)ֲ:(x)ڵĻW(xu)ɷҪwSغ͹z

չ

ټ(x)(x)ڵijɷc|(zh)Y(ji)϶ɵĻ

ڳwSø͹zøȥֲ:(x)

֪R(sh)c(din)Y(ji) 3

1zy(c)߀ԭԇ0.1g/mlNaOHҺ0.05g/mlCuSO4ҺMc߀ԭǰl(f)(yng)ɴutɫʹÕr(sh)עF(xin)F(xin)

2bMе֬KȾҺKȾҺǰߌ֬ȾSɫȾɼtɫ

3bMеĵ|(zh)psԇʹÕr(sh)ȼNaOHҺ2~3CuSO4Һ(yng)ɫj(lu)

4oȾɫwȾɫõȾɫđϻtҺ

5^DNARNAڼ(x)еķֲУü׻G_tɷNȾɫȾɫDNAȾɾGɫRNAȾɼtɫ

6ԭ|(zh)Ӱ(x)ĤҺĤԼ@ɌĤ֮gļ(x)|(zh)

7ǾGȾҺnjһȾwĻ(x)Ⱦϣʹ(x)оwʬF(xin){(ln)Gɫ

8ڷڵ׵ĺϳӹ\(yn)ݔͷ^УP(gun)ļ(x)w(ni)|(zh)W(wng)ߠww

9γҪͨ^Óˮsϵķʽ

10(dng)Һȴڼ(x)Һȕr(sh)ֲ:(x)l(f)|(zh)ڷxF(xin);(dng)ҺСڼ(x)Һȕr(sh)ֲ:(x)l(f)|(zh)ڷxď(f)ԭF(xin)

֪R(sh)c(din)Y(ji) 4

һ W(xu)гҊW(xu)Ԫؼã

1Cawȱ֮(hu)ܛѪҺCa2+͕(hu)鴤^߄t(hu)𼡟oѪҺеCa2+дM(jn)ѪҺ̵ÙccѪҺеCa2+ѪҺͲ(hu)l(f)ֲвٵԪأһȱ۵ĽM(hu)ܵ

2FeѪt׵ĽMɳɷȱ(hu)ȱFؚѪѪtеFeǶr(ji)Fr(ji)FDzõġֲвٵԪһȱ۵ĽM(hu)ܵ

3Mg~GwĽMԪܶøļֲȱVr(sh)~׳F(xin)~}ʧG

4BM(jn)۵Ȱl(f)ͻ۹ܵL(zhng)ȱֲ(hu)F(xin)(sh)

5Iנټصijɷȱ׃(hu)СY˕(hu)طԼנ[

6KѪ⛺^͕r(sh)(hu)F(xin)ļԄ(dng)(ji)ɮ(do)ʧ

7NNǘ(gu)~GATP|(zh)ͺıԪNֲw(ni)γɵĻﶼDz(wn)ĻˮNֲw(ni)Ƅ(dng)ȱNr(sh)~~N(do)~SNһNˮB(ti)ϵy(tng)I(yng)B(yng)һNW(xu)ԪˮB(ti)ϵy(tng)У^NcPϕ(hu)ɸI(yng)B(yng)ڵˮB(ti)ϵy(tng)еĸI(yng)B(yng)Q顰ˮAںB(ti)ϵy(tng)еĸI(yng)B(yng)Q顰ೱ(dng)w(ni)ȱN(sh)Hȱٰ͕(hu)Ӱ푵(dng)wL(zhng)l(f)

8PPǘ(gu)֬ATPıԪֲw(ni)ȱP(hu)Ӱ푵DNAď(f)ƺRNAD(zhun)䛣ĶӰ푵ֲL(zhng)l(f)P߀cֲúͺеf^?yn)ATPADPжPҲˮB(ti)ϵy(tng)I(yng)B(yng)һNԪءֲȱPr(sh)~׳F(xin)o~Gϼtɫt

9ZnijЩøĽMɳɷҲøĻġ߻ͽzϳɫøкZn]ZnͲܺϳȱZnOҵֲС~Yͅ~Y~׃С(ji)gs̡

W(xu)гõԇ

1ԇ ɷ֣0.1g/ml NaOHҺ0.05g/ml CuSO4Һ÷ԇҺҺweϣٌϺԇy(c)ҺˮԡӟֱӼӟᣬy(c)Һд߀ԭtʴutɫ

2Ƕԇ{(ln)ɫҺǻϺˮԡ(hu)F(xin)utɫǵĜy(c)

3psԇɷ֣0.1g/ml NaOHҺ0.01g/ml CuSO4Һ÷y(c)Һȼ2mlҺuм3~4Һuy(c)дڵ|(zh)tʬF(xin)ɫ

4K÷ȡKw95%ľƾУuڙzy(c)֬Ɍ֬ȾSɫKȾɼtɫ

5bDNADNAˮԡ(hu)Ⱦ{(ln)ɫ

6׻GbDNADNA׻Gأ(hu)Ⱦ{(ln)Gɫ

750%ľƾҺ֬bУKȾҺȾɫ50%ľƾҺϴȥɫ

875%ľƾҺښ75%ľƾܝB뼚(x)(ni)ʹ|(zh)׃@(g)ƾĝB͸ÓˮÜp(qing)@(g)t(hu)ʹ(x)浰|(zh)ѸÓˮ̳ĤKƾ͸75ľƾҺg(sh)ǰᘡQˎᘾǰƤwÓԼC(j)е

995%ľƾҺswe?jn)?sh)95%ľƾDNA

1015%}᣺95%ľƾҺweϿڽx

11đҺ(Ȟ0.01g/ml0.02g/ml)ȾɫwɫɌȾɫwȾɫͨȾɫ3~5ҲôtȾɫ

1220%ĸK3%^3.5%ȻFڱ^^øFe3+Ĵ߻ЧrĸKк^ø

133%ĿԵҺ3%Һ2%røҺ̽ø(du)ۺǵÌ(sh)(yn)

14Һb۵Ĵ׃{(ln)

15ͪȡ~Gwеɫ

16Һɷ֣20ʯѡ2ݱͪ1ݱ϶Ҳ93̖(ho)ͣɫصČɫڞVϷx_

17裺ɫصȡķx(sh)(yn)ĥGɫ~Ƭr(sh)ʹĥ֡

18̼}ĥGɫ~Ƭr(sh)룬кЙC(j)ֹĥr(sh)~GwеɫƉ

190.3g/mLҺஔ(dng)30%Һֲ:(x)Һĝȴ|(zh)ڷx(sh)(yn)

200.1g/mLęcҺcuѪѪ

21ȻcҺٿܽDNA(dng)ȻcȞ2mol/L 0.015mol/Lr(sh)DNAܽȻcȞ0.14 mol/Lr(sh)DNAܽڝȞ0.9%r(sh)}ˮ

22ȵøٿÁֽ⵰|(zh)ڿڄ(dng):(x)B(yng)r(sh)ֽMʹM(x)ɢ

23ˮأ˹T(do)౶wԇȰl(f)ķNӻʹȾɫwMӱԭǿڷѵļ(x)Nwγ

24Ȼ}Ӽ(x)(x)ڵͨ͸ԣڻ򹤳̵D(zhun)ʹ(x)̎ڸܑB(ti)

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